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特徴
autoclavable In wet form (pH 7.0) at 120°C for 30 min
包装
pack of 500 g
メーカー／製品名
Cytiva
膨張
1 g swells to 9-11 mL
粒径
100-300 μm (wet)
50-150 μm (dry)
cleaning in place
2-13
working range
2-10
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI Key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
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詳細
Sephadex® G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
特徴および利点
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
- Classic gel filtration medium.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Sephadex is a registered trademark of Cytiva
保管分類コード
13 - Non Combustible Solids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
プロトコル
This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
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