GE11-0034-94

HiTrap^® MabSelect SuRe^™

Cytiva 11-0034-94, pack of 1 × 5 mL

• 別名: HiTrap MabSelect SuRe, MabSelect SuRe Column
• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• ligand: alkali-stabilized protein A-derived (E. coli)
• 包装: pack of 1 × 5 mL
• メーカー／製品名: Cytiva 11-0034-94
• 保管条件: (20% Ehtanol); (20% Ethanol)
• パラメーター: <20 mL/min flow rate; 42 psi
• ベッドサイズ: 16 mm × 25 mm
• ベッド容積: 5 mL
• カラム内径: 16 mm
• マトリックス: highly cross-linked agarose
• 平均部品サイズ: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-13.7
• working range: 3-12
• キャパシティ: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• 適合性: suitable for bioprocess medium
• 保管温度: 2-8°C

詳細

詳細

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ SuRe^™ columns are prepacked with MabSelect^™ SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).

HiTrap^® MabSelect^™ columns are prepacked with MabSelect^™ a bioprocess medium for capuring of Mabs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap^® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

MabSelect SuRe is a trademark of Cytiva

SuRe is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids