G5545

抗β-グルクロニダーゼ (C-末端) ウサギ宿主抗体

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗GUS抗体
• MDL番号: MFCD00282142
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: WB
• クローン: polyclonal
• 化学種の反応性: plant
• citations: 5
• テクニック: western blot: 1-2 μg/mL using purified GUS from E. coli

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 60 kDa
• 化学種の反応性: plant
• 濃度: ~1.5 mg/mL
• テクニック: western blot: 1-2 μg/mL using purified GUS from E. coli
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

β-Glucuronidase (GUS) gene (also referred to as uidA) from Escherichia- coli, codes for a 60kDa protein.

特異性

本抗体は遺伝子導入タバコ植物で発現した細菌のGUSを認識します。

Anit-β-Glucuronidase (C-Terminal) recognizes bacterial GUS expressed in transgenic tobacco plants.

免疫原

E. coli GUSのC末端側アミノ酸589-603に相当する合成ペプチドのKLHに結合体。

アプリケーション

Detection of GUS by immunoblotting (60 kDa). Staining of the GUS band in immunoblotting is specifically inhibited by the immunizing GUS peptide (E. coli, amino acids 589-603).

生物化学的／生理学的作用

β-Glucuronidase (GUS) acts as a reporter gene for plant studies. Reporter genes are widely used for studying the expression of foreign genes in transformed plant tissues. GUS is an hydrolase that catalyzes the cleavage of a variety of β-glucuronide derivatives available for colorimetric, fluorometric and histochemical assays. GUS activity is easily assayed in vitro and can withstand fixation, enabling histochemical localization in cells and tissue sections. However, one of the major limitations of the gus reporter gene system is that the histochemical GUS assay system is destructive for the plant tissue, and therefore it is not suitable for direct visual selection of transformed plants.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

調製ノート

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable