G0907

Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody produced in goat

affinity isolated antibody, lyophilized powder

• 別名: GM-CSF, MGC131935, MGC138897, molgramostin, sargramostim
• MDL番号: MFCD02096490
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: goat
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: lyophilized powder
• 交差性: rat
• テクニック: western blot: 0.1 μg/mL
• UniProtアクセッション番号: P48750
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CSF2(1437) ; mouse ... Csf2(12981) ; rat ... Csf2(116630)

詳細

詳細

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha -helices. Mature rat GM-CSF shares 56-69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha. Rat GM-CSF is active on mouse cells, although mouse GM-CSF is only weakly active on rat cells.

特異性

Detects rat GM-CSF in direct ELISAs and Western blot. In direct ELISAs, approximately 100% cross-reactivity with recombinant feline GM-CSF and recombinant porcine GM-CSF is observed, approximately 30% cross-reactivity with recombinant mouse GM-CSF, recombinant canine GM-CSF and recombinant human GM-CSF is observed.

免疫原

Purified recombinant rat GM-CSF expressed in E. coli.

物理的形状

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline with 5% trehalose

再構成

Reconstitute at 0.2 mg/mL in sterile PBS.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)