F9902

Monoclonal Anti-Fibrinogen antibody produced in mouse

clone 85D4, ascites fluid

• 別名: Anti-Fib2
• MDL番号: MFCD00162408
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 85D4, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: pig, sheep, rabbit, bovine, baboon, human, goat, canine
• テクニック: dot blot: suitable; indirect ELISA: 1:8,000; western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P02671
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FGA(2243) , FGB(2244) , FGG(2266)

詳細

詳細

Fibrinogen is a blood coagulation protein that consists of Aα, Bβ and γ polypeptide chains. Fibrinogen expression is induced by acute-inflammatory responses and has also been associated with the risk of cardiovascular disorders. Monoclonal Anti-Fibrinogen antibody recognizes a conformational epitope, which is destroyed by the cleavage of the γ (302-303) bond. This epitope is found in fibrinogen and in fibrin monomers (devoid of either fibrinopeptide A, or both A and B fibrinopeptides), when they are immobilized on ELISA plates. The antibody reacts with the D-dimer and D-fragment. It does not recognize individual fibrinogen/fibrin chains in denatured-reduced forms or their proteolytic fragments. The E fragment is not recognized by the antibody. The antibody reacts with greater avidity with protofibrils than with wider fibrin fibers in electron microscopic analysis. Monoclonal Anti-Fibrinogen is specific for fibrinogen and fibrin in humans, baboons, bovines, sheep, goats, pigs, rabbits and dogs.

Fibrinogen was the first blood coagulation factor to be identified, and it is a precursor of fibrin protein. The three polypeptide chains are coded by three genes located on human chromosome 4q23-q32, and spans 51kb.

特異性

この製品は、γ(302-303)結合の切断により破壊される高次構造的なエピト-プを認識します。このエピト-プはフィブリノ-ゲンとフィブリンモノマ-の両方に存在しますが、ELISAプレ-ト上に固定したフィブリノペプチドAや、AおよびBフィブリノペプチドには欠けています。本抗体はD-ダイマ-やD-フラグメントと反応します。各フィブリノ-ゲン/フィブリン鎖の変性-還元型や、タンパク質分解フラグメントは認識しません。また、Eフラグメントとも反応しません。電子顕微鏡を使用した場合、抗体は幅広いフィブリン線維よりもプロトフィブリルと強いアビディティ-で反応します。

免疫原

ヒトフィブリン分解産物

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-Fibrinogen antibody is suitable for use in indirect ELISA, dot blot (1:1000 using human fibrinogen), and western blot.

Monoclonal Anti-Fibrinogen antibody produced in mouse has been used in immunohistochemistry and confocal laser scanning microscopy.

生物化学的／生理学的作用

Fibrinogen is an acute-phase inflammatory protein, which plays an essential role in blood clotting.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable