F5392

繊維芽細胞増殖因子-塩基性 ウシ下垂体由来

~90% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

• 別名: FGF2, bFGF
• CAS番号: 106096-93-9
• MDL番号: MFCD00081382
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 製品名: 繊維芽細胞増殖因子-塩基性 ウシ下垂体由来, FGF-Basic, suitable for cell culture
• 由来生物: bovine pituitary glands
• 品質水準: 200
• アッセイ: ~90% (SDS-PAGE and N-terminal analysis)
• フォーム: lyophilized powder
• 有効性: 0.03-3.0 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: 16.4 kDa
• 包装: pkg of 1 μg
• 保管条件: avoid repeated freeze/thaw cycles
• テクニック: cell culture | mammalian: suitable
• 不純物: <0.5 EU/vial
• 色: white
• UniProtアクセッション番号: P03969
• 保管温度: −20°C
• 遺伝子情報: bovine ... FGF2(281161)

詳細

詳細

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

アプリケーション

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:

• In animal and human cell culture
• In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
• In the generation of directly reprogramming fibroblast
• In human glioma cell culture

生物化学的／生理学的作用

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

物理的形状

凍結乾燥品(0.2 μmフィルタ-で濾過したPBS, 塩化ナトリウム(0.1 mg BSA含有))

調製ノート

Gospodarowiczらの方法を改変して腺を精製しました。

アナリシスノート

増殖促進活性は、ウシ胎児心臓内皮細胞で試験されています。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)