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由来生物
goat
結合体
FITC conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
グレード
flow cytometry grade
クローン
polyclonal
形状
buffered aqueous solution
テクニック
flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
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詳細
IgG, a monomer, is the principal antibody circulating in blood which assist in phagocytic destruction of foreign microorganism and is the only class of Ig that can cross the placenta in humans. Cell-sorting done by differential binding of anti-mouse IgG (Fab Specific) - FITC antibody separates tumour cells from macrophages. This antibody can be used to incubate somatic cell nuclear transfer (SCNT) rat embryos for immunocytochemical observation. It also can be used as a secondary antibody to study the dynamics of activated B lymphocytes in small intestine, Peyer′s patch germinal center as well as non-germinal center, spleen and the mesenteric lymph node. Anti-mouse IgG antibody reacts specifically with all mouse immunoglobulins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
特異性
全てのマウスIg分子に結合します。
Fcレセプタ-の存在によるバックグラウンド染色を回避する場合に有用です。
Fcレセプタ-の存在によるバックグラウンド染色を回避する場合に有用です。
免疫原
Purified Fab fragments of mouse IgG.
アプリケーション
Anti-Mouse IgG (Fab Specific) FITC Conjugate antibody is used in traditional method of immunocytochemical assessment of ZAP-70 expression. It can also be used in immunocytochemical observation of the SCNT embryos.
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in:
- indirect immunofluorescence staining
- immunofluorescence staining
- flow cytometry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Flow cytometry/Cell sorting (1 paper)
生物化学的／生理学的作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
その他情報
抗体は、ウシ, ウマ, ヒト血清タンパクで吸収済みです。
物理的形状
0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。
調製ノート
バックグラウンド染色を低減するため、ウシ, ウマ, ヒトサンプルで吸収済みです。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
F2653-BULK:
F2653-.5ML:
F2653-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Agnieszka Bojarska-Junak et al.
Folia histochemica et cytobiologica, 43(1), 19-23 (2005-05-06)
The ZAP-70 protein is a member of the Syk/ZAP protein tyrosine kinase family, normally expressed in T cells and NK cells but not found in normal, mature B cells. The protein plays a critical role in the initiation of T-cell
Gert J Ossenkoppele et al.
British journal of haematology, 162(2), 191-201 (2013-05-08)
Tefinostat (CHR-2845) is a monocyte/macrophage targeted histone deacetylase inhibitor (HDACi). This first-in-human, standard 3 + 3 dose escalating trial of oral, once daily tefinostat was conducted to determine the safety, tolerability, pharmacokinetic and pharmacodynamic profile of tefinostat in relapsed/refractory haematological
Xiaobing He et al.
Stem cells (Dayton, Ohio), 27(7), 1487-1495 (2009-06-23)
Highly tumorigenic cancer cell (HTC) populations have been identified for a variety of solid tumors and assigned stem cell properties. Strategies for identifying HTCs in solid tumors have been primarily empirical rather than rational, particularly in epithelial tumors, which are
The Immunoglobulins: Structure and Function (1998)
C Trollet et al.
Infection and immunity, 77(5), 2221-2229 (2009-02-25)
Botulinum neurotoxins are known to be among the most toxic known substances. They produce severe paralysis by preventing the release of acetylcholine at the neuromuscular junction. Thus, new strategies for efficient production of safe and effective anti-botulinum neurotoxin antisera have
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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