F2047

モノクローナル抗c-Myc抗体-FITC標識 マウス宿主抗体

clone 9E10, purified immunoglobulin, buffered aqueous solution

• 別名: 抗c-Myc
• UNSPSCコード: 12352203
• NACRES: NA.56

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: FITC conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 9E10, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: human
• 保管条件: protect from light
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: 5 μg/mL (suitable for immunoflourescence)
• テクニック: immunocytochemistry: 5.0 μg/mL using mammalian cells transfected with c-Myc tagged fusion protein, fixed with paraformaldehyde.
• UniProtアクセッション番号: P01106
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MYC(4609)

詳細

詳細

Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice. The antibody is isolated from ascites fluid and conjugated to fluorescein isothiocyanate isomer I (FITC). Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain.

特異性

Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein.

免疫原

ヒトp62^c-Mycタンパク質の合成ペプチド。

アプリケーション

Monoclonal Anti-c-Myc−FITC antibody produced in mouse has been used in immunofluorescence microscopy and immunocytochemistry.

生物化学的／生理学的作用

c-Myc plays a vital role in regulation of cell growth, proliferation, differentiation and apoptosis. It is implicated in DNA replication, RNA splicing or transcription. Deregulation in the gene is associated with the development of various types of human cancers. An epitope located within amino acids 410-419, containing the sequence EQKLISEEDL of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tag fusion proteins. Epitope tags provide a method to localize gene products in a variety of cell types, to study the topology of proteins and protein complexes, and to identify associated proteins. In addition, it allows characterization of newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

保管および安定性

For continuous use, store at 2-8 °C for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in “frost-free” freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable