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安全性情報

E4913

Sigma-Aldrich

Plant/Fungi RNA Purification Kit

greener alternative

sufficient for 50 purifications

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About This Item

UNSPSCコード:
41105501
NACRES:
NA.52

使用法

sufficient for 50 purifications

環境により配慮した代替製品の特徴

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

環境により配慮した代替製品カテゴリ

, Aligned

詳細

Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.

アプリケーション

For the rapid purification of total RNA (including microRNA) from plants and fungi.
Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
  • qRT-PCR
  • Reverse transcription PCR
  • Northern blotting
  • RNAse protection
  • Primer extension
  • Expression array assays
  • Next Generation Sequencing

特徴および利点

  • Isolate RNA from a wide range of plant and filamentous fungi samples.
  • Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf.
  • Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA.
  • Purification of high yield and quality RNA.
  • No phenol/chloroform extraction required.
  • Liquid nitrogen is not required for homogenization

その他情報

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

ピクトグラム

Exclamation markEnvironment
GHS07,GHS09

シグナルワード

Warning

危険有害性情報

H302 + H332,H315,H319,H410

危険有害性の分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2

保管分類コード

10 - Combustible liquids

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

キットコンポーネントの情報を参照してください

PRTR

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消防法

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労働安全衛生法名称等を表示すべき危険物及び有害物

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労働安全衛生法名称等を通知すべき危険物及び有害物

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カルタヘナ法

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Jan Code

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試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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オンカラムDNase I消化セット sufficient for 10 preparations

Sigma-Aldrich

DNASE10

オンカラムDNase I消化セット

Yuan Song et al.
Biochemical and biophysical research communications, 396(2), 187-192 (2010-03-25)
DNA methyltransferase2 (DNMT2) is always deemed to be enigmatic, because it contains highly conserved DNA methyltransferase motifs but lacks the DNA methylation catalytic capability. Here we show that Arabidopsis DNA methyltransferase2 (AtDNMT2) is localized in nucleus and associates with histone
Michaela Novodvorska et al.
BMC genomics, 14, 246-246 (2013-04-13)
Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia using both next generation RNA-sequencing and GeneChips. The metabolism of storage compounds during conidial germination was also examined and compared to the transcript levels from associated
Jun-Zhi Wen et al.
PloS one, 9(2), e88179-e88179 (2014-02-12)
MicroRNAs (miRNAs) are important regulators of many cellular processes and exist in a wide range of eukaryotes. High-throughput sequencing is a mainstream method of miRNA identification through which it is possible to obtain the complete small RNA profile of an
Hila Yaffe et al.
BMC research notes, 5, 45-45 (2012-01-21)
Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues
Kobi Buxdorf et al.
Plant molecular biology, 84(1-2), 37-47 (2013-08-15)
The cuticle plays an important role in plant interactions with pathogens and with their surroundings. The cuticle acts as both a physical barrier against physical stresses and pathogens and a chemical deterrent and activator of the plant defense response. Cuticle
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プロトコル

Plant RNA/DNA Purification Kit

The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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