D4692

抗DNMT1抗体 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Anti-DNA methyltransferase
• MDL番号: MFCD06201933
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~180 kDa
• 交差性: human, mouse
• 濃度: 0.8-1.2 mg/mL
• テクニック: microarray: suitable; western blot: 0.5-1.0 μg/mL using nuclear extracts of 293T or HeLa cells
• UniProtアクセッション番号: P26358
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DNMT1(1786)

詳細

詳細

In humans, the gene encodes a DNA (cytosine-5)-methyltransferase with 1616 amino acids. The N-terminal contains the regulatory domain, while the C-terminal region contains the catalytic domain. Two isoforms of DNMT1 have been isolated, DNMT1a and DNMT1b, the difference residing in 16 extra amino acids within the latter.

免疫原

synthetic peptide corresponding to amino acids 72-78 of human DNMT1, conjugated to KLH via an N-terminal added cysteine residue. The sequence is conserved in mouse and is different from the rat sequence by one amino acid.

アプリケーション

Anti-DNMT1 antibody produced in rabbit is suitable for microarray and immunoblotting at a working concentration of 0.5-1.0μg/mL using nuclear extracts of 293T or HeLa cells. It was used at 1:1000 working dilution for immunoblot analysis in a study to analyze whether some miRNAs are aberrantly expressed and target DNMT1 in NiS (nickel sulfide)-transformed cells. It was used for immunoprecipitation of DNMT1 from nuclear lysates of colon cancer cells transfected with DNMT1 vectors in a study. It was used as a primary antibody at a working dilution of 1:1000 for western blot analysis of total protein extracted from left murine testis tissue exposed to low-dose-rate radiation in a study.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)

生物化学的／生理学的作用

DNMT1 protein is important for the maintenance of methylation, as well as for the novo methylation activities occurring in somatic cells of vertebrates. It establishes a repressive transcription complex at replication foci with histone deacetylase HDAAC4 and DMAP1.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)