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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
MEM-122, monoclonal
製品種目
ColorWheel®
フォーム
lyophilized
分子量
calculated mol wt 35.19 kDa
精製方法
affinity chromatography
交差性
human
交差性（ホモロジーによる予測）
nonhuman primates
包装
antibody small pack of 25 μL
環境により配慮した代替製品の特徴
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
テクニック
flow cytometry: suitable
アイソタイプ
IgM
エピトープ配列
Extracellular domain
タンパク質IDアクセッション番号
UniProtアクセッション番号
適合性
for use with ColorWheel® Dyes (Required, (Sold Separately))
環境により配慮した代替製品カテゴリ
, Aligned
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
Monkey ... CD47(103228373)
詳細
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
特異性
Clone MEM-122 is a mouse monoclonal antibody that detects CD47. It targets an epitope within the extracellular domain.
免疫原
COS-7 cells
アプリケーション
Quality Control Testing
Evaluated by Flow Cytometry in A431 cells.
Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye.
Tested Applications
Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye.
Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.
Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Flow Cytometry in A431 cells.
Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye.
Tested Applications
Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye.
Flow Cytometry Analysis: Staining of one million A431 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.
Flow Cytometry Analysis: Staining of one million human peripheral blood lymphocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1143, Anti-Human CD47 (MEM-122) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1017 Mouse IgM isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
ターゲットの説明
Leukocyte surface antigen CD47 (UniProt: A0A0D9R1W9; also known as Integrin-associated protein, CD47) is encoded by the CD47 gene (Gene ID: 103228373) in Monkey. CD47 is a multi-pass transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form. It is widely expressed on all hematopoietic cells and most other cell types. The ligands of CD47 include SIRP , thrombospondin-1 (TSP-1), and integrins including vβ3 and 2β1. It plays a role in vβ3-mediated cell adhesion and Ca2+ influx, as well as in platelet activation via interaction with thrombospondin-1. In addition, CD47 modulates peripheral mononuclear (PMN) neutrophil migration across endothelial and epithelial monolayers. The rate of fMLP-stimulated PMN migration is shown to be decreased by CD47 functional blockage upon neutralizing antibody treatment, which can be partially reversed by PI3K inhibition. Following fMLP stimulation and after PMN transmigration, secondary granules-derived CD47 molecules are shown to accumulate on plasma membrane, resulting in increased cell surface CD47 immunoreactivity. Overexpression of CD47 is reported on the surface of many types of cancer cells where it forms a signaling complex with signal-regulatory protein (SIRP ), enabling the escape of these cancer cells from macrophage-mediated phagocytosis. (Ref.: Zhang, W., et al. (2020). Front. Immunol. 11; 18).
物理的形状
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
再構成
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.
法的情報
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
11 - Combustible Solids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CWA-1143-25UL:
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