T2949

抗mTOR ウサギ宿主抗体

1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗FKBP-12ラパマイシン関連タンパク質抗体, 抗FRAP抗体, 抗RAFT抗体, 抗SEP抗体
• MDL番号: MFCD02263996
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 289 kDa
• 化学種の反応性: rat, human, mouse
• 濃度: 1.0-1.5 mg/mL
• テクニック: immunoprecipitation (IP): 1.0-1.5 μg using 300 μg RIPA extract of human transformed kidney HEK 293T cells; microarray: suitable; western blot: 1:2,000 using whole extracts of rat brain tissue; western blot: 1:500 using mouse brain tissue
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FRAP1(2475) ; mouse ... Frap1(56717) ; rat ... Frap1(56718)

詳細

詳細

mTOR is an evolutionary conserved serine/threonine kinase that belongs to the phosphoinositol kinase-related kinase (PIKK) family. This kinase regulates cell growth by initiation of translation in response to nutrients such as amino acids (primarily leucine), growth factors, mitogens and insulin. Translational initiation is mediated by mTOR through S6K1 activation and 4E-BP1 inhibition. mTOR may also modulate several cellular functions such as actin organization, secretion, membrane trafficking, protein degradation, protein kinase C signaling, tRNA synthesis and ribosome biogenesis. Anti-mTOR antibody is specific for human, mouse and rat mTOR (289 kDa).

特異性

Anti-mTOR (FRAP) is produced using a peptide corresponding to amino acids 2433-2450 of human mTOR. The corresponding sequence is identical in mouse and differs by one amino acid in rat. The antibody specifically recognizes human, mouse and rat mTOR (289 kDa) and is suitable for immunoblotting, immunoprecipitation and immunofluorescence.

免疫原

ヒトmTORのアミノ酸残基2433-2450に対応する合成ペプチド。

The corresponding sequence is identical in mouse and differs by one amino acid in rat.

アプリケーション

Anti-mTOR antibody is suitable for use in Western blot (1:1,000 using myotube cell lysates of rainbow trout cultures). The antibody can also be used in indirect immunofluorescence, immunoprecipitation, and microarray.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable