CWA-1002

Anti-Human CD44 (IM7) ColorWheel^® Dye-Ready mAb

for use with ColorWheel^® Dyes (Required, sold separately)

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: IM7, monoclonal
• 製品種目: ColorWheel^®
• 形状: lyophilized
• 分子量: calculated mol wt 85.62 kDa
• 化学種の反応性: mouse, human
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• テクニック: flow cytometry: suitable
• アイソタイプ: IgG2bκ
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: NP_000601.3
• UniProtアクセッション番号: P16070
• 適合性: for use with ColorWheel^® Dyes (Required, sold separately)
• 環境により配慮した代替製品カテゴリ: Aligned,
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CD44(12505)

詳細

詳細

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

特異性

Clone IM7 is a rat monoclonal antibody that detects CD44.

免疫原

Dexamethasone-induced murine myeloid leukemia M1 cells.

アプリケーション

Quality Control Testing:

Evaluated by Flow Cytometry in mouse splenoctytes.

Flow Cytometry Analysis (FC): Staining of one million mouse splenocytes was performed using 5 μL of 1:1 mixture of Cat. No. CWA-1002, Anti-Human CD44 (IM7) ColorWheel^® Dye-Ready mAb and and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

適合性

Requires ColorWheel^® Dye (Sold Separately)

ターゲットの説明

CD44 antigen (UniProt: P15379; also known as CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44) is encoded by the CD44 (also known as LHR, MDU2, MDU3, MIC4) gene (Gene ID: 12505) in murine species. CD44 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 23-685), a transmembrane domain (aa 686-707), and a cytoplasmic domain (aa 707-778). It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. The main ligand for CD44 is Hyaluronic acid (HA) that is expressed by stromal and cancer cells. HA binds the CD44 ligand binding domain and induces conformational changes that allow binding of adaptor proteins or cytoskeletal elements to intracellular domains. This leads to the activation of various signaling pathways leading to cell proliferation, adhesion, migration, and invasion. Overexpression of CD44 has been reported in several cell types and it is considered as an ideal marker for cancer stem cells. (Ref.: Chen, C., et al. (2018). J. Hematol. Oncol. 11; Article 64).

物理的形状

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

再構成

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法的情報

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable