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製品種目
ColorWheel®
品質水準
形状
lyophilized
環境により配慮した代替製品の特徴
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
テクニック
flow cytometry: suitable
蛍光検出
λex 499 nm; λem 520 nm
適合性
for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)
環境により配慮した代替製品カテゴリ
, Aligned
輸送温度
ambient
保管温度
2-8°C
詳細
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
Alpha Fluor™ 488 NHS Ester (Succinimidyl Ester) is a water soluble, bright green fluorescent dye that exhibits an excitation max at 499 nm and an emission max at 520 nm with an extinction coefficient of 73,000 cm-1 M-1. It is suitable for use over a wide range of pH (4 to 10). It can be used with the 488 nm Argon laser and its spectral properties are identical to Alexa Fluor™ 488. The NHS ester is the most convenient amine-reactive form for conjugating it to proteins and antibodies and can be used for stable signal generation in Flow cytometry. It displays high photostability and brighter fluorescence.
Alpha Fluor™ 488 NHS Ester (Succinimidyl Ester) is a water soluble, bright green fluorescent dye that exhibits an excitation max at 499 nm and an emission max at 520 nm with an extinction coefficient of 73,000 cm-1 M-1. It is suitable for use over a wide range of pH (4 to 10). It can be used with the 488 nm Argon laser and its spectral properties are identical to Alexa Fluor™ 488. The NHS ester is the most convenient amine-reactive form for conjugating it to proteins and antibodies and can be used for stable signal generation in Flow cytometry. It displays high photostability and brighter fluorescence.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
アプリケーション
Flow Cytometry: Excitation: 499 nm, Emission: 520 nm
適合性
Requires ColorWheel® Antibody (Sold Separately)
物理的形状
CWDS-AF488-25UL Set Components
CWD-AF488-25UL - ColorWheel® Antibody-Ready Alpha Fluor 488 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL -ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
CWD-AF488-25UL - ColorWheel® Antibody-Ready Alpha Fluor 488 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL -ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
再構成
Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
法的情報
ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
関連製品
製品番号
詳細
価格
保管分類コード
11 - Combustible Solids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
CWDS-AF488-25UL:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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