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物質
U-bottom
black bottom
black polystyrene
無菌性
non-sterile
特徴
lid: no
skirt
U-bottom (black)
包装
pack of 10
メーカー/製品名
Corning 4514
サイズ
384 well
ウェル容積
35 μL
ウェル
384 wells , low volume
有効容積
2-20 μL
結合型
NBS™ (non-binding surface)
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詳細
The Corning® 384-well microplate, low volume has a skirted, U-bottom design and black polystyrene material. The black walls minimize crosstalk, background luminescence, and fluorescence, resulting in improved luminescent signals. The nonbinding surface (NBS) technology helps reduce molecular interactions at low concentrations thereby increasing assay signal to noise ratio.
アプリケーション
Corning® 384-well microplate has been used for lowering nucleic acid and protein binding at low concentrations while enhancing the assay signal-to-noise ratio.
特徴および利点
- It has a square well that makes robotic pipetting more convenient
- Rounded bottom to reduce trapped air and increased Z factor
- For efficiency, a light cone-shaped conical well is used
- During fluorescent experiments, the presence of opaque walls diminishes autofluorescence and crosstalk between wells
- Microplates have non-ionic hydrophilic surfaces as a result of nonbinding surface technology treatment, which reduces molecular interactions
法的情報
Corning is a registered trademark of Corning, Inc.
NBS is a trademark of Corning, Inc.
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プロトコル
Jump dilution protocol to determine the residence time of a drug (or drug candidate) during its interaction with a kinase using a fluorescence polarization assay based on the detection of ADP.
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