Ishikawa Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 99040201, human uterus, Epithelial-like

• 別名: ISHI Cells, Ishikawa Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human uterus
• 包装: tube of 5 μg 99040201-DNA-5UG; pkg of vial of cells 99040201-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Oestrogen, progesterone
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Asian endometrial adenocarcinoma

細胞株の説明

The cell line Ishikawa was established from an endometrial adenocarcinoma from a 39 year old woman. The cells induced well differentiated adenocarcinoma in athymic nude mice. Estrogen and progesterone receptors were demonstrated both in cell culture and in induced tumours. Ishikawa has been reported to produce corticotropin-releasing hormone, placental alkaline phosphatase and chorionic gonadotrophin, and responded to steroid hormones. The cell line has been eradicated from mycoplasma at ECACC using BM-cyclin. Ishikawa was temporarily removed from the web site whilst an authentication issue was investigated. Following collaboration with key investigators in this field, ECACC is confident that this cell line is Ishikawa.

アプリケーション

Ishikawa Cell Line human has been used to study the effect of 1,25-Dihydroxy-Vitamin D3 (1,25(OH)2D3) on cytosolic pH regulation and glycolytic flux. It has also been used to study endometrial adenocarcinoma.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17

培地

MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; cells tend to pile up.

その他情報

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