EMT6/AR1

96042327, mouse unknown/unspecified, Epithelial-like

• UNSPSCコード: 41106514
• 由来生物: mouse unknown/unspecified
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• 関連疾患: cancer

特性

• 製品名: EMT6/AR1, 96042327
• 由来生物: mouse unknown/unspecified
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse mammary, drug-resistant

細胞株の説明

EMT6/AR1.0 was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in increasing amounts of doxorubicin over a period of 8 weeks. The cell line showed a 40-fold greater resistance to doxorubicin, which is also known as adriamycin. The line was reported to express a classical multi-drug resistance (MDR) phenotype with hyperexpression of membrane P-glycoprotein and a spectrum of cross-resistance e.g. against vincristine and colchicine. Overexpression of CP22 (sorcin) and mdr 1a gene was reported. Cells were shown to have a reduced intracellular drug accumulation and exhibit a doubling time of 12-15 hours. It is recommended to culture the cells without drug after resuscitation until the first passage.

アプリケーション

Drug-resistance studies

培地

MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +1.0 μg/ml doxorubicin + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC. Resuscitate in drug-free medium, add doxorubicin after first passage.

その他情報

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