MCP21

96030418, Reactivity: Anti-Proteasomes, human

• UNSPSCコード: 41106514

特性

• 由来生物: Mouse x Mouse Hybridoma
• イムノドン: BALB/c mouse spleen
• 骨髄腫: Sp2/0
• 反応性: Anti-Proteasomes, human
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse Sp2/0 x Mouse BALB/c spleen

細胞株の説明

Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.

免疫原

Human placenta proteasomes

アプリケーション

Detection and purification of proteasomes and analysis of subunits

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO₂; 37°C

その他情報

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