RIN-5F

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95090402, rat pancreas, Not specified

• UNSPSCコード: 41106514

特性

• 由来生物: rat pancreas
• 包装: tube of 5 μg 95090402-DNA-5UG; pkg of vial of cells 95090402-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Not specified
• 製品: Insulin, L-dopa-decarboxylase
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat islet cell tumour

細胞株の説明

RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (Sigma Catalogue number. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (Sigma Catalogue number. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.

アプリケーション

Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow in small islands and do not form a confluent monolayer.

その他情報

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