DMS 153

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95062827, human lung (liver metastasis), Not specified

• UNSPSCコード: 41106514

特性

• 由来生物: human lung (liver metastasis)
• 包装: tube of 5 μg 95062827-DNA-5UG; pkg of vial of cells 95062827-1VL
• 成長モード: Semi-adherent aggregates
• 核型: Modal no. 41-42
• 形態: Not specified
• 製品: Not specified
• 受容体: Bombesin, Epidermal growth factor (EGF)
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian lung small cell carcinoma, metastasis to liver

細胞株の説明

DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 β-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.

アプリケーション

Human lung tumour model

DNAプロファイル

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 14,15

培地

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.Cells grow in large clumps.

その他情報

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