BF-11

Reactivity: Anti-Erythropoietin, human, 95050413

• UNSPSCコード: 41106514

特性

• 由来生物: Rat x Mouse Hybridoma
• ImmunoDon: Rat AC1 spleen cells
• 骨髄腫: Sp2/0 Ag-14
• 反応性: Anti-Erythropoietin, human
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat ACI spleen x Mouse Sp2/0 Ag-14

細胞株の説明

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

免疫原

Human erythropoietin

アプリケーション

Immunological studies, isolation of erythropoietin by immuno-affinity methods.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

その他情報

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