AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• UNSPSCコード: 41106514

特性

• 由来生物: Horse x Mouse/Horse Hybridoma
• イムノドン: Horse peripheral lymphocytes
• 骨髄腫: NS0
• 反応性: Application: Fusion partner for hybridoma production
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: Not specified
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Horse peripheral blood lymphocytes x Mouse NS0

細胞株の説明

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

免疫原

Influenza A equine 2 virus

アプリケーション

Fusion partner for hybridoma production

培地

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.