LS 123

94120801, human colon, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: LS 123, 94120801
• 由来生物: human colon
• 成長モード: Adherent
• 核型: Modal no. 63 (2n=46), hypotriploid
• 形態: Epithelial
• 製品: Carcinoembryonic antigen (CEA)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human colon adenocarcinoma

細胞株の説明

Established in 1974 from the second in a series of 3 primary colonic tumours resected from a 65 year old Caucasian female, which was classified as a Duke′s B mucin producing colon adenocarcinoma. LS 123 is aneuploid, fails to grow progressively in nude mice and forms colonies only in enriched semisolid medium developed for tumour stem cells. The cells will grow in either low serum or serum free medium. In chick embryonic skin assays the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. LS 123 exhibits several properties associated with neoplasia, but have little invasive potential and may represent an early stage in tumour development.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,13
D16S539: 13
D5S818: 11
D7S820: 9
THO1: 7,8
TPOX: 9
vWA: 16

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat inactivated).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using trypsin 0.25% or trypsin/EDTA; 5% CO₂, 37°C.

その他情報

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