Colo 794

94072237, human skin, Not specified

• UNSPSCコード: 41106514

特性

• 製品名: Colo 794, 94072237
• 由来生物: human skin
• 成長モード: Adherent
• 核型: Not specified
• 形態: Not specified
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human melanoma

細胞株の説明

Colo 794 was established from a 14 year old male patient with a malignant melanoma. The melanoma showed rapid growth and metastases in the patient and the biopsy was taken before therapy from a subcutaneous metastasis. The cell line was eradicated from mycoplasma at ECACC.COLO 794 and COLO 800 (Sigma Catalogue number 93051123) were both derived from the same 14 year old male This has been confirmed by STR profiling.

アプリケーション

Cancer studies

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20

培地

RPMI 1640+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C; cells can show extensive blebs

その他情報

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