F8402

モノクロナール抗CD31 (PECAM-1)−FITC マウス宿主抗体

clone WM-59, purified immunoglobulin, buffered aqueous solution

• 別名: 抗PECAM-1抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: FITC conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: WM-59, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 130-140 kDa
• 交差性: human
• 保管条件: protect from light
• テクニック: flow cytometry: 10 μL using 1 × 10⁶ cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P16284
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PECAM1(5175)

詳細

詳細

CD31 is a single chain integral membrane glycoprotein member of the immunoglobulin superfamily of cell adhesion molecules with molecular mass of 130-140kDa. It has six Ig-like loops in the extracellular domain, a trans-membrane domain including a relatively long cytoplasmic domain. It is expressed abundantly on platelets, myeloid cells, B lymphocytes, certain T-lymphocyte cell subsets, bone marrow precursor cells, NK cells and localized to the intracellular junctions in monolayers of cultured endothelial cells.

Monoclonal Anti-CD31 (PECAM-1) is derived from the WM-59 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特異性

Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025

免疫原

human cell line RC-2A, originally derived from myeloid leukemia cells.

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody has been used in

• DAB based-immunohistochemistry
• direct immunofluorescent staining
• fluorescence-activated cell sorting (FACS)
• flow cytometry

Monoclonal Anti-CD31 (PECAM-1)-FITC antibody is suitable for immunostaining of HEPC-CB2 and HEPC-CB.2 cell Lines (hematopoietic cells) and flow cytometry to evaluate the percentage of contaminating endothelia cells in characterization and decidualization of endometrial stromal cells.

生物化学的／生理学的作用

CD31 is also known as PECAM-1 (Platelet endothelial cell adhesion molecule-1) binds to glycosaminoglycans (GAG). Endothelial CD31 is phosphorylated on tyrosine and serine residues. CD31 is involved in some of the interactive events taking place during cardiovascular development inflammation, thrombosis, wound healing and angiogenesis.

CD31 performs in homophilic and heterophilic cell-cell adhesion and cell signalling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix.

ターゲットの説明

Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.

物理的形状

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

調製ノート

フルオレセインイソチオシアネ-トアイソマ-I(FITC)とのコンジュゲ-ションにより作製しています。この緑色色素は励起波長495 nm, 蛍光波長525 nmでの測定が効果的です。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)