DSL6A/C1

94022431, rat pancreas, Polygonal

• UNSPSCコード: 41106514

特性

• product name: DSL6A/C1, 94022431
• 由来生物: rat pancreas
• 成長モード: Adherent
• 核型: Not specified
• 形態: Polygonal
• 製品: Expresses CFTR protein
• 受容体: Lacks CCK-A receptors
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat pancreatic carcinoma

細胞株の説明

DSL6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an azaserine treated Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumours composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting.

アプリケーション

Study of pancreatic carcinomas, differentiation of acinar/ductal cells.

培地

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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