TGP 52

94022429, mouse pancreas, Trabecular

• UNSPSCコード: 41106514

特性

• product name: TGP 52, 94022429
• 由来生物: mouse pancreas
• 成長モード: Adherent
• 核型: Not specified
• 形態: Trabecular
• 製品: Somatostatin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse islet cell tumour

細胞株の説明

TGP 52 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E) Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 52 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of pancreatic tumour lines deposited at ECACC. Cells transiently produce insulin in culture followed by somatostatin.

アプリケーション

Study of endocrine pancreas carcinomas, hormone assays.

培地

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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