TGP 49

94022427, mouse pancreas, Trabecular

• UNSPSCコード: 41106514

特性

• product name: TGP 49, 94022427
• 由来生物: mouse pancreas
• 成長モード: Adherent
• 核型: Not specified
• 形態: Trabecular
• 製品: Somatostatin (low levels)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse pancreatic acinar carcinoma

細胞株の説明

TGP 49 has been isolated from an acinar cell carcinoma arising in the pancreas of a transgenic Tg(E1a-1-SV40E)Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 49 secretes low amounts of somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. They have been reported not to secrete amylase or lipase but do secrete trypsin. Differentiation characteristics fluctuate from time to time in culture. Trabecular and clumps of small cells.

アプリケーション

Study of endocrine pancreas carcinomas, hormone assays.

培地

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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