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製品名
EZZ (TOU II-4), 93093003
由来生物
human blood
成長モード
Suspension
核型
Not specified
形態
Lymphoblast
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Human B cell lymphocyte, EBV transformed
細胞株の説明
Peripheral blood lymphocytes from a male Tunisian, immortalised by EBV transformation. The cell line contains the following deletions, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4.
培地
RPMI 1640 + 2mM グルタミン + 10% ウシ胎児血清(FBS)
継代と培養方法
Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.
その他情報
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