NIH 3T3 Cell Line murine

93061524, mouse embryo, Fibroblast

• 別名: 3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells
• UNSPSCコード: 41106514

特性

• 製品名: NIH 3T3 Cell Line murine, 93061524
• 由来生物: mouse embryo
• 詳細: Embryonic fibroblast
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse Swiss NIH embryo

細胞株の説明

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.

アプリケーション

DNA transfection studies

NIH 3T3 Cell Line has been used to:

• study calcium-mediated actin reset (CaAR) in response to physiological changes
• study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
• determine the viral titer of the retroviral construct -ST40hTNFα

培地

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

その他情報

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