HT29 Cell Line human

91072201, human colon, Epithelial

• 別名: HT29 Cells
• UNSPSCコード: 41106514

特性

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• 由来生物: human colon
• 成長モード: Adherent
• 核型: 2n = 46, hypertriploid
• 形態: Epithelial
• 製品: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian colon adenocarcinoma

細胞株の説明

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

アプリケーション

発がん性の研究

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

培地

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

その他情報

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