WI 38 Cell Line human

90020107, human lung (fetal), Fibroblast-like

• 別名: WI38 Cells, Wistar Institute-38 Cells
• UNSPSCコード: 41106514

特性

• product name: WI 38 Cell Line human, caucasian fibroblast-like fetal lung cell, 90020107
• 由来生物: human lung (fetal)
• 成長モード: Adherent
• 核型: 2n = 46, diploid except at high passage number
• 形態: Fibroblast-like
• 製品: Collagen
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian foetal lung

細胞株の説明

From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.

アプリケーション

継代培養:70～80%コンフルエントの状態で、約1:3～1:6に分割してください(2～4 × 10⁴生細胞/cm²で播種)。37°C、5% CO₂で0.25%トリプシン溶液(EDTA含有または不含)を用いて処理してください。

From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.

Virus studies (rhinoviruses), vaccine production

WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.

生物化学的／生理学的作用

Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.

アナリシスノート

Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS

その他情報

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