175BR

90011863, human skin, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: 175BR, 90011863
• 由来生物: human skin
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human skin fibroblast

細胞株の説明

Established from an adult male with primary carcinoma. The donor was the brother of the donor of 174BR (Sigma Catalogue number. 90011895).

アプリケーション

DNA repair studies

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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