Clone C6/36

89051705, insect larvae, Not specified

• UNSPSCコード: 41106514

特性

• 製品名: Clone C6/36, 89051705
• 由来生物: insect larvae
• 成長モード: Adherent
• 核型: Diploid
• 形態: Not specified
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mosquito larvae, Aedes albopictus

細胞株の説明

Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.

アプリケーション

Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO₃) level of 1.1g/l is recommended).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm² . Cells are dislodged by mechanical agitation; 5% CO₂; 28°C. Freeze in 10% glycerol.

その他情報

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