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製品名
Clone C6/36, 89051705
由来生物
insect larvae
成長モード
Adherent
核型
Diploid
形態
Not specified
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Mosquito larvae, Aedes albopictus
細胞株の説明
Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.
アプリケーション
Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.
培地
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO3) level of 1.1g/l is recommended).
継代と培養方法
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm2 . Cells are dislodged by mechanical agitation; 5% CO2; 28°C. Freeze in 10% glycerol.
その他情報
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