SAOS-2 Cell Line human

89050205, human bone, Epithelial-like

• 別名: SAOS2 Cells
• UNSPSCコード: 41106514

特性

• 製品名: SAOS-2 Cell Line human, 89050205, primary osteogenic sarcoma
• 由来生物: human bone
• 包装: tube of 5 μg 89050205-DNA-5UG; pkg of vial of cells 89050205-1VL
• 成長モード: Adherent
• 核型: 2n = 46, (P1) Hyperploid to hypopentaploid
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human primary osteogenic sarcoma

細胞株の説明

Reported to have been derived from an 11 year old female Caucasian. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. HLA cell line phenotype: A2,3;Bw16,w47.

アプリケーション

SAOS-2 cell line has been used to study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA). It has also been used to study the effect of chromium-enriched diamond-like carbon (DLC) layers on cell adhesion and proliferation.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 18

培地

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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