P22

89030317, hamster ovary, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: P22, 89030317
• 由来生物: hamster ovary
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Hamster Chinese ovary

細胞株の説明

Chinese hamster ovary cell line which lacks ornithine decarboxylase activity.

培地

EMEM (EBSS) + Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% BSA.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.1% cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 min. or until cells detach; 5% CO₂; 37°C. Pre-treat culture flasks with porcine gela

その他情報

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