NCTC clone 929

88102702, mouse connective tissue, Fibroblast

• 別名: Earles′s Cells, L Cells, L-929 Cells, NCTC 929 Cells, NCTC-929L Cells
• UNSPSCコード: 41106514

特性

• product name: NCTC clone 929, 88102702
• 由来生物: mouse connective tissue
• 成長モード: Adherent
• 核型: 2n = 40
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse C3H/An connective tissue

細胞株の説明

From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.

アプリケーション

Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.

その他情報

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