HeLa S3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87110901, human cervix, Epithelial

• 別名: HeLa-S3 Cells, HeLa/S3 Cells, HeLaS3 Cells, S3 HeLa Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human cervix
• 包装: tube of 5 μg 87110901-DNA-5UG; pkg of vial of cells 87110901-1VL
• 成長モード: Suspension
• 核型: Modal no. 68
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human cervix carcinoma

細胞株の説明

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.

アプリケーション

HeLa S3 has been used to study the cytotoxicity of dinuclear titanium-salan complexes. It has also been used to perform in vitro genotoxicity tests (comet and micronucleus) to study the effects of occupational exposure to carcinogens on sewage workers.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培地

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS)

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Culture in a spinner flask. This cell line may adhere if the culture is not agitated.

その他情報

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