BF-2

87032603, fish trunk, Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: BF-2, 87032603
• 由来生物: fish trunk
• 包装: tube of 5 μg 87032603-DNA-5UG; pkg of vial of cells 87032603-1VL
• 成長モード: Adherent
• 核型: 2n = 48
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Fish Bluegill fry caudal trunk

細胞株の説明

Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15°C to 33°C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

アプリケーション

Virus studies, targets for bacterial cytotoxins

培地

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells

その他情報

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