Ehrlich-Lettre Ascites strain E cell line

87032503, mouse unknown/unspecified, Epithelial

• UNSPSCコード: 41106514

特性

• product name: Ehrlich-Lettre Ascites strain E cell line, from mouse unknown/unspecified, 87032503
• 由来生物: mouse unknown/unspecified
• 成長モード: Adherent
• 核型: 2n = 40
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse carcinoma

細胞株の説明

Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.

アプリケーション

Anti-tumour test and tumourigenicity studies

培地

NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change 3 times a week.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.