LS180

87021202, human colon, Epithelial-like

• UNSPSCコード: 41106514

特性

• product name: LS180, 87021202
• 由来生物: human colon
• 成長モード: Adherent
• 核型: Modal no. 45 (2n=46)
• 形態: Epithelial-like
• 製品: Carcinoembryonic antigen (CEA), Mucin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian colon adenocarcinoma

細胞株の説明

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

アプリケーション

発がん性の研究

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

その他情報

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