SC-1

86060301, mouse embryo, Fibroblast

• UNSPSCコード: 41106514
• 由来生物: mouse embryo
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified

特性

• 製品名: SC-1, 86060301
• 由来生物: mouse embryo
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse feral embryo

細胞株の説明

Sensitive to both N- and B- trophic viruses. Cells exhibit a greater sensitivity than other mouse lines to MuLV strains and to a limited extent support the replication of xenotropic MuLV. This cell line has been mycoplasma eradicated at ECACC.

アプリケーション

Virus studies: N- and B- trophic viruses, MuLV

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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