NIH-3T3 4-2

86041101, mouse skin, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: NIH-3T3 4-2, 86041101
• 由来生物: mouse skin
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

詳細

Product has lost contact inhibition

細胞株の由来

Mouse fibroblast

細胞株の説明

A mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.

アプリケーション

Virus studies: Moloney MuLV, retroviruses

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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