BRL 3A

85111503, rat liver, Epithelial

• UNSPSCコード: 41106514

特性

• product name: BRL 3A, 85111503
• 由来生物: rat liver
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: MSA factors
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Buffalo Rat liver

細胞株の説明

Originally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).

アプリケーション

Growth factor studies

培地

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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