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由来生物
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
骨髄腫
Sp2/0
反応性
Anti-c-Myc gene product, human
テクニック
cell culture | mammalian: suitable
アイソタイプ
IgG1, kappa
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Mouse Sp2/0 x Mouse BALB/c spleen
細胞株の説明
The antibody recognises human p62c-myc gene product and is specific for C-terminal peptide G. The antibody failed to immunoprecipitate p62 c-myc, but recognised the imunoblotted protein. Compared to other hybridomas specific to this antigen (Mycl-8F9, Mycl-3C7, CT9-B7, CT14-B2) Mycl-9E10 is the most sensitive probe for immunoblots. Conformation of antigen is critical for antibody recognition. No reported cross-reaction, with chicken p110gag-myc protein or mouse c-myc protein. This cell line is available as a growing culture only to customers in Europe.
免疫原
A synthetic peptide, corresponding to residues 408-432(C-terminus of Peptide G) of human p62c-myc sequence, conjugated to KLH
培地
RPMI 1640 + 2mM グルタミン + 10% ウシ胎児血清(FBS)
継代と培養方法
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are slow to grow. This cell line is available as a growing culture only to customers in Europe.
その他情報
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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