WI 38VA13 Subline 2RA

85062512, human lung (fetal), Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: WI 38VA13 Subline 2RA, 85062512
• 由来生物: human lung (fetal)
• 成長モード: Adherent
• 核型: 2n = 46, hyperdiploid, modal no. 73-78
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian foetal lung, SV40 transformed

細胞株の説明

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

アプリケーション

Transformation and virus studies: SV40

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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