HT115

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85061104, human colon, Epithelial

• 別名: HT-115 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human colon
• 包装: tube of 5 μg 85061104-DNA-5UG; pkg of vial of cells 85061104-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human colon carcinoma

細胞株の説明

Cells possess Ca antigen as defined by the Ca1 antibody. The Ca antigen has been shown to be absent in non-malignant human cell strains and is considered to be a marker linked with malignancy. Island cell growth will degenerate if left more than 9 days between passages.

アプリケーション

発がん性の研究

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 7,12
D13S317: 13
D16S539: 10
D5S818: 12
D7S820: 11,12
THO1: 6,9
TPOX: 8
vWA: 18

培地

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells grow relatively slowly, in islands.

その他情報

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