CHO Cell Line from Chinese hamster ovary

85050302, hamster ovary, Epithelial

• 別名: CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
• UNSPSCコード: 41106514

特性

• product name: CHO Cell Line from Chinese hamster ovary, 85050302, from hamster ovary
• 由来生物: hamster ovary
• 利用可能性: not available in Japan
• 成長モード: Adherent
• 核型: Hypodiploid, modal no. 20
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Hamster Chinese ovary

細胞株の説明

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

アプリケーション

CHO Cell Line from Chinese hamster ovary has been used:

• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

培地

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.