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product name
EL4 Cell Line murine, 85023105, Lymphoblast from blood
由来生物
mouse blood
成長モード
Suspension
核型
2n = 39
形態
Lymphoblast
製品
A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Mouse ascites lymphoma lymphoblast
細胞株の説明
Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen histone-2b (H-2b), interleukin-4 (IL-4) and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to polyhydroxyalkanoates (PHAs).
アプリケーション
発がん性の研究
EL4 Cell Line murine has been used to induce tumor in mice and to assess the extravasation of tumor necrosis factor (TNF)-induced Evans blue dye and E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc). It has also been used as a constituent of bone marrow-derived macrophage (BMDM)-polarizing media to polarize BMDM and assess its tumoricidal activity.
培地
DMEM + 2mM グルタミン + 10% ウシ胎児血清(FBS)
継代と培養方法
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth after resuscitation is slow. Subculture once exponential growth is established.
その他情報
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試験成績書(COA)
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