MDCK

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011435, dog kidney, Epithelial-like

• 別名: MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSCコード: 41106514
• eCl@ss: 32190102

特性

• 由来生物: dog kidney
• 包装: tube of 5 μg 85011435-DNA-5UG; pkg of vial of cells 85011435-1VL
• 成長モード: Adherent
• 核型: 2n = 78
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Canine Cocker Spaniel kidney

細胞株の説明

Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).

アプリケーション

Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

その他情報

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